BIOL 201 - Cellular and Molecular Biology
This course will address the fundamental concepts of Cellular and Molecular Biology. Lectures, assignments, and laboratory projects will explore the structure and function of molecules, organelles, and cells and the biological processes they are involved in. Students in this course will gain an understanding of various molecular mechanisms, structure/function relationships, and cellular processes as they relate to cellular and molecular biology. Students in this course will practice and carry out common laboratory techniques used by Cellular and Molecular Biologists including, recombinant DNA technology, cell trafficking, and cloning techniques.
BIOL 403 - Fundamentals of Plant Biochemistry and Pathology
This course is primarily focused on biochemical and pathological aspects of plants. This course provides an understanding of why protein catalysts are important in the field of plant biochemistry and plant pathology. More specifically, the role enzymes play in the basic cellular processes of plant growth and development is presented. Topics related to plant pathology are presented; such as plant disease epidemics, plant diagnosis, plant diseases caused by fungi, bacteria, nematodes, viruses, and plant-pathogen interaction, at the ecological, physiological and genetic level.
BIOL 401 - Biological Separations: Principles and Practices
This is a laboratory-based course that teaches classic concepts and techniques to enable the use of these techniques to purify small molecules and macromolecules from whole organisms. Detection techniques will include the use of bacterial biosensors, coomassie-blue staining, silver staining, and immunoblot analysis. Separation techniques will include SDS Polyacrylamide gel electrophoresis (PAGE) analysis, thin layer chromatography, and paper electrophoresis. Purification techniques will include ammonium sulfate precipitation, affinity chromatography, and thin layer chromatography.
BIOL 345 - Molecular Ecology
This course explores the biology of populations and communities of organisms using molecular data. As DNA, RNA and proteins are nearly universal between organisms, the principles taught in this course will have wide applications, both within ecology and throughout many sub-disciplines of biology. Furthermore, this course will prepare students to apply the techniques in numerous research fields. The primary literature and worldwide applications of the field of molecular ecology will be incorporated into the course.
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André O. Hudson, Ph.D.
Thomas H. Gosnell School of Life Sciences
Rochester Institute of Technology
85 Lomb Memorial Drive
Rochester, NY 14623
Office: Gosnell Hall (Bldg 08) Room A358
Lab: Gosnell Hall (Bldg 08) Room A355
The relative map positions of aminotransferase and aminotransferase-like genes distributed on the 5 chromosomes of Arabidopsis thaliana
Transamination interconverts pairs of amino acids and keto acids. During transamination, the amino group of an amino acid is transferred to a keto acid, this produces a new keto acid while from the original keto acid, a new amino acid is formed. The enzyme employs a ping-pong (double displacement)(see cartoon above) mechanism facilitated by the co-factor pyridoxal phosphate (PLP) which is bound to a conserved lysine residue in the active site of the enzyme.