You can complete the following take home problem and turn in your results by
e-mail or on a paper copy when you turn in your report.
To begin this project, you will need to download and install Ionex.zip.
This file contains the program, help files, some protein files and some sample
separation files (these end in .iex). It works best if all the files are installed
in the directory "C:\ionex". You will need to use Winzip
to extract these files. In addition to the ionex program, the zip file also
contains the files for the four proteins you will be studying:
Glycogen phosphorylase (filename = 1pyg.pdb)
Hemoglobin (filename = 1hga.pdb)
cathepsin (filename = 1the.pdb)
Tumor suppressor P53 (filename = 1tup.pdb)
To receive credit, you will need to do the following:
Select conditions that will separate the four proteins into four separate peaks
on the chromatogram. Conditions include the buffer selected, salt concentration
in solvent A and solvent B and stationary phase. You can either write these
on a piece of paper, or save your designed experiment using the "Save as"
button on the Design Experiment window.
Identify the isoelectric point for each of the four proteins using the molecular
view window. You need to adjust the pH bar to get the charge as close to 0.0
as possible. Report the pH value that you have found. The scale on the slider
bar has 14 tick marks for pH values ranging from 1 to 14
Questions or problems regarding this web site should be directed to pacsch@rit.edu.
Copyright © 1999-2003 by Paul A. Craig. All rights reserved.
Last modified: Tuesday, March 25, 2003.