Ion Exchange Chromatography

Ion exchange chromatography is a separation based on charge. This web site is designed to simulate the process of ion exchange chromatography and to help you learn to design an ion exchange separation. You will be able to practice selecting initial conditions for the mobile phase (buffer, pH and salt concentration) and the stationary phase (cation or anion exchanger). You will also learn how to design a mobile phase gradient to achieve the best separation.

The stationary phase has either positively or negatively charged species immobilized on its surface. Positively charged stationary phases are called anion exchangers because anions bind to them. Negatively charged stationary phases are called cation exchangers.

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