Stationary Phase

The stationary phase provides the basis for the separation in chromatography and usually consists of the separating principle (charge, hydrophobic character, affinity ligand) chemically bound to an insoluble resin or gel. The stationary phase is packed in the chromatography column. When a protein is applied to the column, it equilibrates between the stationary phase and the mobile phase as it passes through the column. Proteins that are more tightly bound to the stationary phase require a stronger mobile phase to elute them from the column.

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