Void Volume

The void volume is the volume of mobile phase (V_m or V₀) in a column. For example, if the stationary phase occupies 40% of the total column volume, the void volume would be 60% of the total column volume.

Consider a column that is 25 cm long with an inner diameter of 1 cm. The total column volume is 19.6 mL (V = p r²L = 3.14 * 0.5² * 25 cm). If the mobile phase occupies 60% of the column volume, the void volume is 11.8 mL.

Related Topics

Chromatography

Column