Dr. Robert Rothman

 Office: 08-1342
Phone: 475-5215
E-Mail: rhrsbi
web site: people.rit.edu/rhrsbi
 

Teaching Assistants:

Monday/Wednesday Labs

Tuesday/Thursday Labs

Matt Woodruff

Access to BioDoc-It:

ftp://biodocit@129.21.189.9

user name = biodocit
no password or account necessary

 
     

Lab Computer Access:

log in to: Biolab1213 (this computer)
user name = gelab
password = ecoli



Important Dates:

Exam 1: April 2
Exam 2: April 23
Exam 3: May 20, 10:15-12:15

 

Texts:

Gene Cloning & DNA Analyss, 5th ed. T.A. Brown . Blackwell Scientific Publications

Calculations for Molecular Biology and Biotechnology. F.H. Stephenson. Academic Press

Genetic Engineering Lab Manual, 5th ed R.H. Rothman Rochester Institute of Technology Press

Plasmids:

pUC9
pPL608

65 micrograms per ml
75 micrograms per ml

  

Laboratory
Cleanup:

Each student is responsible for helping to keep lab clean. Regardless of how you find the lab when you come in, you must swab your workplace down with detergent before and after lab, and you must empty the pipet disposal in the sink into the glass waste disposal. You must also clean up after yourself whenever you use the balance.

In addition to your personal responsibilities, you will be assigned to a clean-up crew according to the schedule below. Responsibilities of the clean-up crew are:

  • Set out microcentrifuges at beginning of class and return them at the end
  • Return balances to stockroom and tidy up balance areas
  • Clean transilluminator
  • Turn off water baths
  • Empty any pipet containers that classmates did not empty
  • Gather up any equipment or reagents that must be returned to instructor
  • Help instructor put away any additional apparatus or reagents

Clean-up Crew assignments by seat location:

 4 4   4 4   4 4
 3 3   3 3   3 3
 2 2   2 2   2 2
 1 1   1 1   1 1

Front

On-Line Lab Manual

Lecture Topics

Grading Criteria

Fomat for Lab Notebooks

Format For Lab Reports

Qiagen HiSpeed Plasmid Purification Handbook

Exam File

Experiment 1 Gel

Ligation Gel

Transformation Plates

Plasmid Screen Gel

Southern Blots

The Ducky Duddle Song

Dr. Rothman's Home Page