Genetic Engineering On-Line Laboratory Manual
Cover
Table of Contents
About Genetic Engineering
Laboratory Resources
Digestion of DNA with Restriction Endonucleases
Agarose Gel Electrophoresis
Restriction Mapping
Experiment 1: Analysis of Plasmid DNA by Restriction Digestion and Agarose Gel Electrophoresis
Experiment 2: Effect of Agarose Concentration on Migration of DNA Fragments
The First Lab Report
Experiment 3: Insertion of a Gene for Chloramphenicol Resistance from
Bacillus subtilis
into an
Escherichia coli
Plasmid
Experiment 4: Transformation of
Escherichia coli
With a Chimeric Plasmid
Experiment 5: Screening of Transformants for Chimeric Plasmids
Experiment 6: Large -Scale Purification of plasmids pRIT4501 and pRIT4502 by Cesium Chloride Density Gradient Centrifugation
Experiment 7: Large -Scale Purification of plasmids pRIT4501 and pRIT4502 by Qiagen Ion Exchange Column Chromatography
Experiment 8: Verification of Purified Plasmids
Experiment 9: Construction of a Restriction Map of Plasmids pRIT4501 and pRIT4502
The Second Lab Report
Experiment 10: Transformation of Plasmids pRIT4501 and pRIT4502 into
Bacillus Subtilis
Experiment 11: Heterologous Gene Expression in
Escherichia coli
and
Bacillus subtilis
The Third Lab Report
Experiment 12: Southern Blot Analysis of Plasmids pRIT4501 and pRIT4502
The Fourth Lab Report
Appendix I: Index of Reagent Formulas &
Appendix II: Shorthand DNA Structure
Appendix III: Cloning Methodology (not available as PDF)
