On-Line Laboratory Manual 7th ed.
Cover
Lab Manual In Single PDF File (152 pages)
Table of Contents
About Genetic Engineering
Laboratory Resources
Digestion of DNA with Restriction Endonucleases
Agarose Gel Electrophoresis
Restriction Mapping
Experiment 1: Analysis of Plasmid DNA by Restriction Digestion and Agarose Gel Electrophoresis
Experiment 2: Effect of Agarose Concentration on Migration of DNA Fragments
The First Lab Report
Experiment 3: Insertion of a Gene for Antibiotic Resistance from Bacillus subtilis into an Escherichia coli Plasmid
Experiment 4: Transformation of Escherichia coli With a Chimeric Plasmid
Experiment 5: Screening of Transformants for Chimeric Plasmids
Experiment 6: Large -Scale Purification of plasmids pRIT4501 and pRIT4502 by Cesium Chloride Density Gradient Centrifugation
Experiment 7: Large -Scale Purification of plasmids pRIT4501 and pRIT4502 by Qiagen Ion Exchange Column Chromatography
Experiment 8: Verification of Purified Plasmids
Experiment 9: Construction of a Restriction Map of Plasmids pRIT4501 and pRIT4502
The Second Lab Report
Experiment 10: Transformation of Plasmids pRIT4501 and pRIT4502 into Bacillus Subtilis
Experiment 11: Heterologous Gene Expression in Escherichia coli and Bacillus subtilis
The Third Lab Report
Experiment 12: Southern Blot Analysis of Plasmids pRIT4501 and pRIT4502
The Fourth Lab Report
Appendix I - VII
Appendix I: Index of Reagent Formulas
Appendix II: Shorthand DNA Structure
Appendix III: Conversion Factors
Appendix IV: Buffer Dilutions
Appendix V: Bacillus subtilis Plasmid Prep by Qiagen Maxi Kit
Appendix VI: Effect of BAP Treatment on Transformation Frequencies
Appendix VII: Bacteriophage Lambda