Dr. Robert Rothman

Office: 08-1342
Phone: 475-5215
E-Mail: rhrsbi
web site: people.rit.edu/rhrsbi
 

Teaching Assistants:

 

 

Aizan
  Athirah
 

Nor Athirah
Ahmadtarmizi
(Athirah)


Access to BioDoc-It:

ftp://cos-azure.rit.edu

user name = azureuser
password = AzureBiology

 
     

Important Dates:

Exam 1: 21 February
Exam 2: 30 March
Exam 3: 18 April
Exam 4: 15 May 10:15 - 12:15: GOS 2178


Texts:

Principles of Gene Manipulation and Genomics 7th ed.
SB Primrose and RM Twyman
Blackwell Publishing.

Genetic Engineering Lab Manual, semester ed.
R.H. Rothman
Rochester Institute of Technology Press


Modifications to Experiments
9 and 11:

Experiment 9:
     Cut pUC9 and pBAC with HindIII

Experiment 11:
   
Use PstI to screen recombinant plasmid


Plasmids:

pUC9
pBAC
pRHR30
pRHR53

211.4
282.8
122.7
98.0


 micrograms per ml
 micrograms per ml
 micrograms per ml
 micrograms per ml

On-Line Lab Manual

Syllabus

Grades To Date

Lecture Topics

Grading Criteria

Format for Lab Notebooks

Format For Lab Reports

Qiagen HiSpeed Plasmid Purification Handbook

Exam File


Lecture Shockwave Files


Experiment 1 Lecture

DNA Structure and UV Absorption Lecture (shockwave file)


Typical Data


Experiment 3: Chemical Nature of DNA (Thin Layer Chromatogram)

Experiment 4: DNA Sequence Specificity Gel

Experiment 5: DNA Nucleases (Endo- and Exonuclease Gel)

Experiment 5: DNA Nucleases (Mung Bean Nuclease Gel)

Experiment 6: Restriction and Modification Gel

Experiment 7 Plasmid Analysis Gel

Ligation Gel

Transformation Plates

Plasmid Screen Gel

Southern Blots


Lab Reports


Lab Report 1: DNA Characterization

Lab Report 2: Action of Nucleases


The Ducky Duddle Song

Dr. Rothman's Home Page